English (United Kingdom)

https://curated-unify.zendy.io/wp-json/zendy-region/v1/featured_content/oa?rat=en

https://curated-unify.zendy.io/wp-json/zendy-region/v1/highlighted_journal/

Global: Zendy Plus annual

Presents the access of premium content as premium feature

Premium Content

Presents the keyphrase highlighting as premium feature

Keyphrase Highlighting

Presents the summarisation as premium feature

Summarisation

Insights

Presents the pdf analysis as premium feature

PDF Analysis

Presents the zaia usage as premium feature

ZAIA

Global: Zendy Tools annual

Global: Zendy Free Plan

Global: Zendy Tools monthly

Global: Zendy Plus monthly

The carbapenemase-encodingbla KPC gene, which is rapidly spreading in Gram-negative rods, is located on a Tn3 -based transposon, Tn4401 , which carries a polymorphic region giving rise to five isoforms (a ,b ,c ,d , ande ) that is located immediately upstream of thebla KPC gene and thus likely involved in its expression. Using 5′ rapid amplification of cDNA ends (5′RACE), we identified three potential promoter sequences (P1, P2, and P3) upstream of thebla KPC gene, of which only P1 (absent from isoformsc andd ) and P2 (present in all isoforms, with a −35 box located inside the right inverted repeat of ISKpn7 ) were shown to be true promoters involved in expression. One representative of each different promoter combination of Tn4401 , i.e., P2 alone (isoformc ), P1-P2 (isoforma ), and P1-P2-P3 (isoformb ), was cloned into anEscherichia coli plasmid vector. Using reverse transcription-PCR (RT-PCR), the highest level of expression was obtained with isoforma (P1 and P2), which is also the most commonly encountered form in enterobacterial clinical isolates, followed by isoformsb (P1, P2, and P3) andc (P2 only). These differences in expression led to slight differences in MIC values of carbapenems.In silico analysis of the DNA sequence of isoformb revealed a stem-loop structure that is likely responsible for strong stops observed in 5′RACE experiments and for decreased expression compared to that with isoforma (P1 and P2). In addition, such structures could also be at the origin for the deletions observed in isoformsa andc . Taken together, these results indicate that the P1 and P2 promoters both contribute to the expression of thebla KPC gene and that the construct with the highest level of expression is that possessing isoforma , which is also the most commonly encountered form in clinical isolates.

Role of IS Kpn7 and Deletions in bla KPC Gene Expression

Role of IS Kpn7 and Deletions in bla _KPC Gene Expression