Open AccessCoexistence of Two bla NDM-5 Genes on an IncF Plasmid as Revealed by Nanopore Sequencing
Author(s) -
Yu Feng,
Lu Liu,
Alan McNally,
Zhiyong Zong
Publication year - 2018
Publication title -
antimicrobial agents and chemotherapy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.07
H-Index - 259
eISSN - 1070-6283
pISSN - 0066-4804
DOI - 10.1128/aac.00110-18
Subject(s) - plasmid , biology , gene , sequence (biology) , genetics , escherichia coli , gene duplication
In a carbapenem-resistantEscherichia coli clinical isolate of sequence type 167, two copies ofbla NDM-5 were found on a 144,225-bp IncF self-transmissible plasmid of the F36:A4:B− type. Bothbla NDM-5 genes were located in 11,065-bp regions flanked by two copies of IS26 . The two regions were identical in sequence but were present at different locations on the plasmid, suggesting a duplication of the same region. This study highlights the complex genetic contexts ofbla NDM-5 .
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