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Klebsiella pneumoniae isolates frequently contain complex mixtures of bla(SHV) alleles. A high-resolution melting-based method for interrogating the extended-spectrum activity conferring codon 238 and 240 polymorphisms was developed. This detects minority extended-spectrum beta-lactamase-encoding alleles, allows estimation of allele ratios, and discriminates between single and double mutants.

Analysis of bla SHV Codon 238 and 240 Allele Mixtures Using Sybr Green High-Resolution Melting Analysis

Analysis of bla _SHV Codon 238 and 240 Allele Mixtures Using Sybr Green High-Resolution Melting Analysis