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Integrons are genetic elements that can acquire and rearrange gene cassettes. Thebla BEL-1 gene encodes an extended-spectrum β-lactamase, BEL-1, that is present at the second position of the variable region of class 1 integrons identified inPseudomonas aeruginosa . The mobility of thebel-1 gene cassette was analyzed under physiological conditions and with the integrase gene being overexpressed. Cassette mobility inEscherichia coli was detected by excision/integration into the recipient integron In3 on the conjugative plasmid R388 with the overproduced integrase. Despite several antibiotic pressures, thebel-1 cassette remained at the second position in the integron, highlighting its stability inP. aeruginosa . Overexpression of the integrase gene inE. coli inducedbel-1 cassette recombination. However, cassettes containing two genes (bla BEL-1 andsmr2 orbla BEL-1 andaacA4 ) were excised, suggesting that thebel-1 cassetteattC site was defective. We show thatbel-1 is a stable gene cassette under physiological growth conditions, irrespective of the selective antibiotic pressure, that may be mobilized upon overexpression of the integrase gene.

Integrase-Mediated Recombination of the bel-1 Gene Cassette Encoding the Extended-Spectrum β-Lactamase BEL-1