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Phosphorylation on tyrosine residues is recognized as an important mechanism for connecting extracellular stimuli to cellular events and defines a variety of physiologic responses downstream of G protein-coupled receptor (GPCR) activation. To date, few protein tyrosine phosphatases (PTPs) have been shown to associate with GPCRs, and little is known about their role in GPCR signaling. To discover potential cysteinyl-leukotriene receptor (CysLT 1 R)-interacting proteins, we identified protein tyrosine phosphatase ε (PTP ε ) in a yeast two-hybrid assay. Since both proteins are closely linked to asthma, we further investigated their association. Using a human embryonic kidney cell line 293 (HEK-293) cell line stably transfected with the receptor (HEK-LT1), as well as human primary monocytes, we found that PTP ε colocalized with CysLT 1 R in both resting and leukotriene D4 (LTD 4 )-stimulated cells. Cotransfection of HEK-LT1 with PTP ε had no effect on CysLT 1 R expression or LTD 4 -induced internalization, but it inhibited LTD 4 -induced CXC chemokine 8 (CXCL8) promoter transactivation, protein expression, and secretion. Moreover, reduced phosphorylation of extracellular signal regulated kinase 1/2 (ERK1/2), but not of p38 or c-Jun-N-terminal kinase 1 or 2 mitogen-activated protein kinases (MAPKs), was observed upon LTD 4 stimulation of HEK-LT1 coexpressing cytosolic (cyt-) PTP ε , but not receptor (R) PTP ε The increased interaction of cyt-PTP ε and ERK1/2 after LTD 4 stimulation was shown by coimmunoprecipitation. In addition, enhanced ERK1/2 phosphorylation and CXCL8 secretion were found in LTD 4 -stimulated human monocytes transfected with PTP ε -specific siRNAs, adding support to a regulatory/inhibitory role of PTP ε in CysLT 1 R signaling. Given that the prevalence of severe asthma is increasing, the identification of PTP ε as a new potential therapeutic target may be of interest.

Role of Protein Tyrosine Phosphatase Epsilon (PTPε) in Leukotriene D4-Induced CXCL8 Expression

Role of Protein Tyrosine Phosphatase Epsilon (PTPε) in Leukotriene D₄-Induced CXCL8 Expression