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A differential phase contrast (DPC) method is validated for in vivo human retinal and choroidal vasculature visualization using high-speed swept-source optical coherence tomography (SS-OCT) at 1060 nm. The vasculature was identified as regions of motion by creating differential phase variance (DPV) tomograms: multiple B-scans were collected of individual slices through the retina and the variance of the phase differences was calculated. DPV captured the small vessels and the meshwork of capillaries associated with the inner retina in en face images over 4 mm2 in a normal subject. En face DPV images were capable of capturing the microvasculature and regions of motion through the inner retina and choroid.

In vivo human retinal and choroidal vasculature visualization using differential phase contrast swept source optical coherence tomography at 1060 nm