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This paper reports on some new results from the analyses of the videomicroscopy data obtained in a prior experiment on two-dimensional (2D)colloidal crystals. It was reported previously that optical tweezers can beused to create mono- and di-vacancies in a 2D colloidal crystal. Here we reportthe results on the creation of a vacancy-interstitial pair, as well astri-vacancies. It is found that the vacancy-interstitial pair can belong-lived, but they do annihilate each other. The behavior of tri-vacancies ismost intriguing, as it fluctuates between a configuration of bound pairs ofdislocations and that of a locally amorphous state. The relevance of thisobservation to the issue of the nature of 2D melting is discussed.

Optical Tweezers as a Micromechanical Tool for Studying Defects in 2D Colloidal Crystals