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At present, the microscopic visualization of luminescent labels containing lanthanide(III) ions, primarily europium(III), as light-emitting centers is best performed with time-gated instrumentation,which by virtually eliminating the background fluo- rescence results in an improved signal to noise ratio. However, the use of the europium(III) macrocycle, Quantum DyeF2emission,of the Eu-macrocycles was observed as an extremely,sharp band with a maximum,at 619 nm and a clearly resolved characteristic pattern. At very low Eu-macrocycle concentrations, another sharp emission was detected at 614 nm, arising from traces of Eu(III) present in even the purest commercially available gadolinium,products. Discrimination of the resolved emissions of the Eu-macrocycle and Eu(III) contaminant should provide a means to further lower the limit of detec- tion of the Eu-macrocycle. Keywords:Luminescence, lanthanide, macrocycle, europium, gadolinium, cofluorescence.

<title>Addition of a second lanthanide ion to increase the luminescence of europium(III) macrocyclic complexes</title>