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Optical diffraction tomography (ODT) is an interferometric microscopytechnique capable of measuring 3-D refractive index (RI) distribution oftransparent samples. Multiple 2-D holograms of a sample illuminated withvarious angles are measured, from which 3-D RI map of the sample isreconstructed via the diffraction theory. ODT has been proved as a powerfultool for the study of biological cells, due to its non-invasiveness, label-freeand quantitative imaging capability. Recently, our group has demonstrated thata digital micromirror device (DMD) can be exploited for fast and precisecontrol of illumination beams for ODT. In this work, we systematically studythe precision and stability of the ODT system equipped with a DMD and presentmeasurements of 3-D and 4-D RI maps of various types of live cells includinghuman red blood cells, white blood cells, hepatocytes, and HeLa cells.Furthermore, we also demonstrate the effective visualization of 3-D RI maps oflive cells utilizing the measured information about the values and gradient ofRI tomograms.

Optical diffraction tomography using a digital micromirror device for stable measurements of 4-D refractive index tomography of cells