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An explicit dosimetry model has been developed to calculate the apparent reacted 1  O  2 concentration ([ 1  O  2 ] rx ) in an in-vivo model. In the model, a macroscopic quantity, g , is introduced to account for oxygen perfusion to the medium during PDT. In this study, the SOED model is extended for PDT treatment in phantom conditions where vasculature is not present; the oxygen perfusion is achieved through the air-phantom interface instead. The solution of the SOED model is obtained by solving the coupled photochemical rate equations incorporating oxygen perfusion through the air-liquid interface. Experiments were performed for two photosensitizers (PS), Rose Bengal (RB) and Photofrin (PH), in solution, using SOED and SOLD measurements to determine both the instantaneous [ 1 O 2 ] as well as cumulative [ 1 O 2 ] rx concentrations, where [ 1 O 2 ]  rx  = (1/ τ  Δ ) · ∫[ 1 O 2 ] dt . The PS concentrations varied between 10 and 100 mM for RB and ~200 mM for Photofrin. The resulting magnitudes of [ 1 O 2 ] were compared between SOED and SOLD.

A feasibility study of singlet oxygen explicit dosmietry (SOED) of PDT by intercomparison with a singlet oxygen luminescence dosimetry (SOLD) system