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We present a method for quantitative phase recovery using the axially defocused intensity information based on the phase optical transfer function in defocused situation. The image formation process is linearized by subtraction of two intensity images with equal and opposite defocus distances and quantitative phase information is separated and extracted by solving an inverse problem with Wiener filtering. Experiments confirm the accuracy and stability of the proposed method outperforms the transport-of-intensity reconstruction method. © 2013 SPIE.

Quantitative phase from defocused intensity by image deconvolution