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mechanisms that may involve complex molecular diffusion dynamics, Journal of Biomedical Optics 10(3), 034007 (May/June 2005)as well as information about structural changes that a cellular fluoro-phore undergoes in the course of cell activation. © 2005 Society of Photo-Optical Instrumentation Engineers. [DOI: 10.1117/1.1924712] Keywords: fluorescence lifetime (FLT); fluorescence intensity (FI); fluorescence an-isotropy (FA); rotational correlation time; fluorescence anisotropy decay (FAD); fre-quency domain time resolved microscope (FDTRM). Paper 04028 received Mar. 1, 2004; revised manuscript received Sep. 30, 2004; accepted for publication Dec. 1, 2004; published online Jun. 7, 2005.

Tracing apoptosis and stimulation in individual cells by fluorescence intensity and anisotropy decay