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The anti-biofilm effect of a newly developed antibacterial coating, a mixture of a silver citrate, soybean lecithin and liquid silicon at the ratio of 2:2:8, for silicon catheters was investigated in an in vitro experimental system using a modified Robbins device. The inhibitory effect against bacterial adherence, killing effect against adhered bacteria and durability of these anti-biofilm effects were examined using two strains of Pseudomonas aeruginosa and one of Escherichia coli. Almost complete inhibition of bacterial adherence was demonstrated in the case of one of two strains of P. aeruginosa and E. coli. The more resistant strain of P. aeruginosa OP 14-210 adhered at scattered sites of the silicon with antibacterial coating, while mature biofilm developed on the surface of the silicon without coating. Using already adhered P. aeruginosa OP 14-210, we confirmed the killing effect of silver ion released by this new coating. These anti-biofilm effects did not change after treatment with artificial urine for at least 48 hours.

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Evaluation of the Anti-biofilm Effect of a New Anti-bacterial Silver Citrate/Lecithin Coating in an In-vitro Experimental System Using a Modified Robbins Device