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A chemical component has been purified from Acholeplasma laidlawii which binds to tissue culture cells, MOLT-4, Hut-78, but not MT-4 and Jurkat. The glycolipid in the membranes of A. laidlawii was extracted by Bligh-Dyer method. Further purification of chloroform phase of Bligh-Dyer method was performed by silicagel column chromatography and thin layer chromatography. Finally, the active component was assigned to be diglucosyl diacylglyceride by using nuclear magnetic resonance (1H, 13C). Furthermore, diglucosyl diacylglyceride(s) with C14 and C18 were synthesized, by the method of Boom. Both native and synthesized diacylglycerides bind to MOLT-4 and Hut-78 cells. The binding activity of these substances to cells was inhibited by preincubation of diglucosyl diacylglycerides on the cover glass with clathrin. These results suggest that the binding site of diglucosyl diacylglycerides on cells was clathrin. It is necessary to clarify the biological activities of diglucosyl diacylglycerides in viral infections and transmission of lipoprotein and the how mechanism of envelopment of the virus into the cell.

The Binding Activity of Diglucosyl Diacylglyceride Derived from Membranes of Acholeplasma laidlawii PG8 to Lymphoid Cells