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We found a substance A in human serum by ELISA method, using anti-A antibody. The substance A was obtained from the membranes of A. laidlawii and this enhanced HIV replication. This substance in human serum was detected in the chloroform phase of the Bligh-Dyer method. Rf value in thin layer chromatography was 0.28. This substance clearly showed enhancement of HIV replication as well as the activity of the membranes of A. laidlawii, but did not induce TNF production. Furthermore, the activity which enhances HIV replication in human serum was reduced by anti-A antibody. These results suggest that the substance derives from human serum which possesses a similar active site as those of substance derived from the membrane of A. laidlawii.

A Factor to Enhance HIV Replication in Serum Bearing a Resemblance to Membranes of Acholeplasma laidlawii