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Mensik L., Kostkova E., Kulhavý J. 2015: The effect of thinning on humus conditions in spruce and beech stands in the Highlands area of the Czech Republic. - Beskydy, 8 (2): 101-110 in the stands of spruce. The C/N ratio in the layers of forest floor and soil horizons at each site is similar and is not statistically significantly different. In spruce and beech stands in layers F and H fulvic acids prevail - a lower HA to FA ratio, the transformation of humic substances is more complete in layer H under spruce stands compared with beech. In the Ah horizon, the situation is reversed, with the transformation of humic substances being more complete under beech stands. The results incorporated using multivariate statistical methods (PCA) confirmed that thinning has no statistically significant effect on humus conditions in young spruce and beech stands in different types of habitats.