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Palat M. Sr., Palat M. Jr., J. Prudký J. 2013: Modelling of natural water retention in the catchment basin of the Opava river during flood. - Beskydy, 6 (2): 109-116 The aim of the analysis involved in the article is to define the significance of separate factors affecting the natural process of retention in the catchment basin thus can assess the priorities in undertaking flood protection. The analysis was based on results achieved by using a new method how to determine water retention in the catchment basin. Resulting dependent variables of effective long-time retention, effective short-time retention, effective total retention and maximum specific runoff for single catchment basins of the Opava river, were evaluated statistically by means of linear regression and correlation analysis, stepwise regression and multiple regression. It is possible to say, that the results achieved may suggest to re- evaluate some established views concerning the role of separate factors influencing natural retention of the landscape. The results of the research testify the fact that in case of densely settled floodplain regions the combined flood protection measures, i.e. as technical and nature friendly ones should be applied. Due to relatively small collection of observations it is not possible to generalize all the conclusions absolutely, nevertheless they have a certain validity at least for the given area.