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ŠTÝBNAROVÁ MARIE, LÁTAL OLDŘICH, HLADKÝ JAN, HRADILOVÁ MONIKA, SKLÁDANKA JIŘÍ, BILOŠOVÁ HANA, BRTNICKÝ MARTIN. 2018. Changes in Grassland Chemical Soil Parameters Four Years after Cessation of Different Fertilisation with Compost and Slurry. Acta Universitatis Agriculturae et Silviculturae Mendelianae Brunensis, 66(1): 0211 – 0218. The aim of this study was to evaluate changes in chemical soil properties of permanent grasslands after the cessation of their regular utilisation and organic fertilisation. A long‐term small plot trial was established in 2004 in locality Rapotín. During 2004 – 2012 the experiment was fertilised with compost and slurry, both with the range of stocking rates 0.9, 1.4, and 2.0 livestock units (LU).ha−1 (corresponding to 54, 84, and 120 kg N.ha−1). It was further observed the unfertilised grassland as control. The plots were cut 2 – 4 times per year depending on given dose of fertiliser. During 2013 –2016 the regular management was ceased and the grasslands were completely abandoned. It was found statistically significant influence of the year and the type of fertiliser almost for all evaluated parameters. The dose of nitrogen was not significant. On the basis of our results we can conclude, that the both types of the organic fertilisers had a positive influence on the chemical soil properties, however, the compost manifested itself as the better fertiliser than the slurry from this point of view. In 2016, four years after the last application of the organic fertilisers, there were found the better chemical soil conditions in the treatments previously fertilised with compost.