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DŽELETOVIĆ ŽELJKO, MIHAILOVIĆ NEVENA. 2017. Available Nitrogen in the Surface Mineral Layer of Natural Meadows. Acta Universitatis Agriculturae et Silviculturae Mendelianae Brunensis, 65(5): 1483 – 1492. Based on a greenhouse experiment, we evaluated nitrogen availability in the surface mineral layer of soil under various natural meadow stands by analyzing the following soil characteristics: total organic C, total N, initial content of easily available N inorganic forms, mineralized N content obtained by aerobic and anaerobic incubations and A-value. The experiment was performed on a test plant and through the application of urea enriched with 5.4 % 15N. The investigated soils under natural meadows are characterized with comparatively high mineralization intensity and high N availability indices.