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POLCAR ADAM, CUPERA JIŘI, KUMBAR VOJTĚCH. 2016. Calibration and its Use in Measuring Fuel Consumption with the CAN-Bus Network. Acta Universitatis Agriculturae et Silviculturae Mendelianae Brunensis, 64(2): 503–507. The article deals with the description of calibration and its use in measuring fuel consumption via the CAN-Bus network. The CAN-Bus communication network holds great potential for the area of vehicle testing, since it allows the utilization of internal sensors of the vehicle to monitor its output parameters. In order to ensure proper accuracy of the measurements, however, it is best to perform calibration of the relevant sensors using certifi ed measuring instruments. The main focus of the article is the calibration of the fuel consumption as measured by the CAN-Bus network based on fuel consumption measured by precision mass fl ow meters. Using the least squares method and a regression triplet, a calibration curve was created from the measured data to describe the relation between the two variables. The calibration curve was also used to calculate the reverse estimates along with confi dence intervals for randomly chosen fuel consumptions obtained from the CAN-Bus network. To express the accuracy of the calibration methods, we also defi ned the limit values which express when the signal is still statistically signifi cant enough to be distinguished from noise.