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SOCHOREC, M., KNOT, P.: The eff ect of fertilizer seed coating on the germinating capacity and initial development of some turf grass species and white clover. Acta univ. agric. et silvic. Mendel. Brun., 2012, LX, No. 5, pp. 199– 204 The eff ect of the iSeed method treatment on germination dynamics, total germinating capacity and the length of above-ground and root parts was studied in laboratory conditions for the Red fescue varieties Darwin and Moccasin, Kentucky bluegrass varieties Yvette and Miracle, Perennial ryegrass varieties Beatrice and Milan, and White clover varieties Pirouette and Pipolina. In the Perennial ryegrass, the iSeed treatment adversely aff ected the energy of germination in the fi rst days of study. The germinating capacity gradually equalized during the experiment. Red fescue and Kentucky bluegrass did not show any response to the treatment. Similarly as the Perennial ryegrass, the two varieties of White clover exhibited higher energy of germination on the third day from the establishment. A demonstrably positive eff ect of the iSeed treatment was recorded on the number of germinated individuals. Particularly the variety Pirouette showed signifi cantly higher germinating capacity until the tenth day of monitoring the treated seeds. The iSeed method had no statistically signifi cant infl uence on the values of total germinating capacity in all tested species and varieties. The eff ect of the iSeed method treatment on the length of shoot and root parts was not statistically demonstrated. Some varieties showed a positive eff ect and some showed a negative eff ect. A signifi cant diff erence was observed only in the untreated variety Moccasin whose above-ground part was by 14 mm longer as compared with the treated variant. seed treatment, germinating capacity, iSeed, turf species, microclover Germination and emergence rate of grasses a er sowing are important indicators of the competitive capacity of grasses aff ecting development and character of the grass stand. The capacity of seeds to germinate fast and evenly is a basic prerequisite for the successful establishment of the turf. Turfs are currently established with using seeds that are treated by various methods in order to enhance their fi eld germination and competitiveness as compared with the untreated seeds. One of such methods is iSeed, which is based on the coating of seeds with fertilizer. According to Nijëstein (2008), the used coat layer contains 20% of nitrogen and 4% of phosphorus. Nitrogen is partly in the readily soluble form and partly in the slowly soluble form; phosphorus is only in the fast release form. Černoch (2009) informs that by contrast to the breeding of varieties focused on the improvement of genetic characteristics of plants, new seed treatment technologies modify conditions for the emergence of seeds so that the potential of the variety can be fully used. By using the iSeed method, we can supply up to 40 kg of fertilizer per one hectare of the sown area. The advantage of this method consists in easier sowing, better contact with soil; if a damaged turf has to be repeatedly sown, naked grains penetrate better through the above-ground part of the sward and are thus protected from being blown away by wind or eaten by birds (Knot et al., 2008). 200 M. Sochorec, P. Knot Recently, seed producers have started to focus on alternative species for grass mixtures those are more resistant to stress conditions (drought and trampling). One of such species is the increasingly used White clover ‘Microclover’, which forms minute leafl ets not disturbing the turf appearance. MATERIAL AND METHODS The experiment was established at the Research Station of Fodder Crops in Vatín in 2011. The eff ect of the treatment of naked seeds by the iSeed method on the dynamics of germination, total germinating capacity and the length of above-ground and root phytomass was studied in laboratory conditions for the Red fescue (Festuca rubra ssp. commutata ‘Darwin’ and Festuca rubra ssp. trichophylla ‘Moccasin’), Kentucky bluegrass (Poa pratensis ‘Yvette’ and ‘Miracle’), Perennial ryegrass (Lolium perenne ‘Beatrice’ and ‘Milan’) and White clover (Trifolium repens ‘Pipolina’ and ‘Pirouette’). The experimental variants are presented in Tab. I. The treated and untreated seed used in the experiment was of the same batch. For each variant, twenty seeds were placed on Petri dishes with moistened fi lter paper in three repetitions. Strips of fi lter paper provided for moisture in the dishes. The trial was established on the TZ 8-046 germinator with the adjusted day regime of 16 hours at 25 oC (light on) and the night regime of 8 hours at 15 oC (light off ). The number of germinated seeds was counted on Day 3, 7, 10, 13, 16 and 20 from the establishment. Upon the end of the experiment on Day 20, fi ve largest individuals were selected from each variant in order to measure the length of their above-ground and root parts. Because the germination results were in the form of relative abundance and the detected percentage values could not be expressed by means of Gaussian distribution, a conversion was made by means of the ArcusSinus square root transformation (Gomez and Gomez, 1984). Signifi cance was tested by Anova (Statistika, Version 8) and by the subsequent Tukey test (p ≤ 0.05). RESULTS AND DISCUSSION Results of our experiment with the grass species (Tabs. II, III and IV) show that the treatment of seeds by the iSeed method increased the values of total germinating capacity by 2.3% (Red fescue var. Darwin), 7.6% (Kentucky bluegrass var. Yvette) and 4.3% (Perennial ryegrass var. Beatrice). However, the diff erences in germinating capacity were in all cases insignifi cant. Determining the eff ect of the treatment of naked seeds of meadow grass varieties Yvette and Miracle by using the Proradix method, Knot (2010) observed an insignifi cantly increased germinating capacity, too, and the treatment of Kentucky bluegrass naked seeds with Headstart and Pregerm methods did not have a signifi cant eff ect on the germinating capacity either (Knot, 2006). On the other hand, the total germinating capacity was lower in the treated Red fescue var. Moccasin (−7.1%), Kentucky bluegrass var. Miracle (−8%) and Perennial I: Experimental variants

The effect of fertilizer seed coating on the germinating capacity and initial development of some turf grass species and white clover