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PŘIDAL, A., SVOBODA, J.: Queen introduction into the queenright honey bee colony. Acta univ. agric. et silvic. Mendel. Brun., 2010, LVIII, No. 5, pp. 307–312 One of the actual elementary biologic principles of the introduction of queen is that the recipient colo ny has to be queenless. We accidentally found that a queen can be accepted also in queenright colo ny with using of the queen excluder. Therefore, we conducted two experiments with the introduction of queen in queenright colony. Under defi ned conditions of the experiment and with application of the queen excluder as a separator of queens we successfully introduced queen in the queenright colony. This result is discussed in relation to the general principle that a queen should be introduced only in a queenless colony. It is possible that there are some exceptions advert to the existence of some unknown biologic patterns in the honey bee biology and pheromones. Apis mellifera, honey bee, ethology, beekeeping, introduction of queen, polygyny