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PAVLOUSEK, P.: Mapping of the occurrence of grapevine viruses: GFLV and ArMV in vineyards of the grapevine breeding station Polesovice. Acta univ. agric. et silvic. Mendel. Brun., 2007, LV, No. 5, pp. 117–122 Due to their worldwide distribution and also detrimental potential virus diseases of grapevine are economically very important. In grapevine plants Grapevine fanleaf virus (GFLV) and Arabis mosaic virus (ArMV) is one of the most harmful virus pathogens. The aim of this study was evaluate the occurrence of GFLV and ArMV in vineyards of the Grapevine Breeding Station Polesovice (Czech Republic) using the ELISA test and visual symptoms. In every year from 2001–2004 altogether 225 grapevine plants were tested for the occurrence of GFLV and ArMV. In case of GFLV positive results were obtained in 43 plants, i. e. in 19.11% of all samples. In case of ArMV there were 51 positive tests, i.e. 22.66%. Our results demonstrated a occurrence of GFLV and ArMV in propagation vineyards, which was comparable with results obtained abroad. It was also demonstrated that a professional visual selection could provide a good basis for the identifi cation of virus diseases and their subsequent tests. grape variety, grapevine virus disease, GFLV, ArMV, ELISA method, visual symptoms Grapevine virus diseases are of a high economic importance because they occur worldwide and can cause great damages in vineyards. The most important virus diseases of grapevine plants can be classifi ed into two major genera: Nepoviruses and Closteroviruses. The Arabis mosaic virus (ArMV) and the Grapevine fanleaf virus (GFLV), two nepoviruses, are both causative agents of the fanleaf disease of grapevine, one of most damaging virus diseases affecting grapevines (Wetzel et al., 2002). Grapevine fanleaf virus (GFLV), is degeneration, which causes poor berry set and a yield loss, which exceed 80% in some grapevine varieties. Grapevine viruses can cause severe losses by substantially reducing yield, affecting fruit quality and shortening the lifespan of infected plants in vineyard. (Rakhshandehroo et al., 2005). GFLV is transmitted from grapevine to grapevine by ectoparasitic nematode Xiphinema index (AndretLink et al., 2004). Grafting of cuttings on infected rootstocks may result in a reduced yield of graftings. In Germany for example, the yield of Riesling grafting on healthy and GFLV-infected SO 4 rootstocks was 30–45% and 6– 10%, resp. (Bruckbauer, 1962). As compared with healthy vineyards, yield losses obtained in GFLV-infected stands may be as much as 80% (Rudel, 1983). ELISA has been as a reference method for routine virus diagnosis of grapevine (Anfoka et al., 2004). GFLV can be detected routinely by enzyme-linked immunosorbent assay of various grapevines extracts (leaves collected during spring, rootlets, cortical scrapings from mature canes, petioles) (Andret-Link, et al., 2004).

Mapping of the occurrence of grapevine viruses: GFLV and ArMV in vineyards of the grapevine breeding station Polešovice