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The feasibility of Citrobacter freundii A1 and Enterococcus casseliflavus C1 bacterial consortium under sequential facultative anaerobic-aerobic treatment for complete dye degradation using AR-27 dyes shows 98% decolourisation and 100% COD removal after 72 hours treatment. Moreover, the UV-Vis spectroscopy and Fourier Transform Infrared (FTIR) Spectroscopic analysis confirmed that the azo linkage was cleaved after the decolourisation occurred. While, the cyclic voltammetry analysis also shows that the decolourisation of AR-27 by C. Freundii A1 and E. Casseliflavus C1 was an irreversible reaction and the detection of oxidation reaction under agitation proved the presences of AR-27 degradation process. Furthermore, the HPLC analysis has confirmed the AR-27 degradation through the decrease in catechol concentration.

Biodecolourisation of Acid Red 27 Dye by Citrobacter freundii A1 and Enterococcus casseliflavus bacterial consortium