Open AccessA differentially methylated region of the DAZ1 gene in spermatic and somatic cells
Author(s) -
Li ZuoXiang,
Ma Xu,
Wang ZhaoHui
Publication year - 2006
Publication title -
asian journal of andrology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.701
H-Index - 74
eISSN - 1745-7262
pISSN - 1008-682X
DOI - 10.1111/j.1745-7262.2006.00069.x
Subject(s) - methylation , somatic cell , cpg site , biology , dna methylation , gene , promoter , azoospermia , sperm , genetics , microbiology and biotechnology , differentially methylated regions , gene expression , infertility , pregnancy
Aim: To investigate the methylation status of the deleted in azoospermia 1 ( DAZ1 ) gene promoter region in different cell types. Methods: Using CpG island Searcher software, a CpG island was found in the promoter region of the DAZ1 gene. The methylation status of this region was analyzed in sperm and leukocytes by bisulfited sequencing. Results: The methylation status of the CpG island in the DAZ1 gene promoter region differed in leukocytes and sperm: it was methylated in leukocytes, but unmethylated in sperm. Conclusion: A differentially methylated region of the DAZ1 gene exists in spermatic and somatic cells, suggesting that methylation of this region may regulate DAZ1 gene expression in different tissues.