Open AccessTwo multiplex sets of eight and five microsatellite markers for the European corn borer, Ostrinia nubilalis Hübner (Lepidoptera: Crambidae)
Author(s) -
DALECKY AMBROISE,
BOGDANOWICZ STEVEN M.,
DOPMAN ERIK B.,
BOURGUET DENIS,
HARRISON RICHARD G.
Publication year - 2006
Publication title -
molecular ecology notes
Language(s) - English
Resource type - Journals
eISSN - 1471-8286
pISSN - 1471-8278
DOI - 10.1111/j.1471-8286.2006.01410.x
Subject(s) - ostrinia , european corn borer , biology , crambidae , microsatellite , lepidoptera genitalia , genetics , pyralidae , genotyping , locus (genetics) , allele , botany , gene , genotype
Primer sequence and polymorphism data are presented for 13 microsatellite loci isolated from the European corn borer moth, Ostrinia nubilalis , as part of a project to construct a linkage map for the two pheromone strains. Experimental conditions are described for polymerase chain reaction (PCR) multiplexing, which allows genotyping in two electrophoresis runs of eight and five markers each. In a sample of 27 individuals coming from one European locality, the number of alleles per locus ranged from one to 12, and gene diversity from 0 to 0.859. Seven loci showed a deficit of heterozygotes. Eleven loci cross‐amplify in the related Ostrinia furnacalis .
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