Open AccessVertical distribution of bacterioplankton in L ake A verno in relation to water chemistry
Author(s) -
Paganin Patrizia,
Chiarini Luigi,
Bevivino Annamaria,
Dalmastri Claudia,
Farcomeni Alessio,
Izzo Giulio,
Signorini Antonella,
Varrone Cristiano,
Tabacchioni Silvia
Publication year - 2013
Publication title -
fems microbiology ecology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.377
H-Index - 155
eISSN - 1574-6941
pISSN - 0168-6496
DOI - 10.1111/1574-6941.12048
Subject(s) - biology , bacterioplankton , bacteroidetes , betaproteobacteria , temperature gradient gel electrophoresis , deltaproteobacteria , water column , species richness , firmicutes , proteobacteria , alphaproteobacteria , methanomicrobiales , ecology , gammaproteobacteria , 16s ribosomal rna , botany , actinobacteria , bacteria , genetics , phytoplankton , methanosarcina , methane , methanogenesis , nutrient
Abstract The effects of environmental factors on bacterioplankton distribution along the water column of L ake A verno ( N aples, I taly) have been investigated by means of denaturing gradient gel electrophoresis ( DGGE ) of polymerase chain reaction ( PCR )‐amplified 16 S r RNA gene fragments, and multivariate analysis applied to molecular data and physico‐chemical parameters. Bacterial richness, estimated from DGGE profiles, remains constant throughout the water column, whereas archaeal richness increases with depth. Moreover, archaeal richness was significantly correlated to most of the measured abiotic variables, whereas bacterial richness did not. Analysis of sequences from DGGE bands revealed that the dominant bacterial populations belong to A lphaproteobacteria , B etaproteobacteria , D eltaproteobacteria , B acteroidetes and F irmicutes, whereas sequences of the archaeal DGGE bands are affiliated to M ethanomicrobiales and M ethanosarcinales orders. Moreover, bacterial sequences affiliated to P roteobacteria and B acteroidetes do not group closely to typical freshwater lineages/clades/tribes within these phyla. Bacterioplankton distribution along the water column was apparently correlated with the vertical gradient of physico‐chemical parameters as a statistically significant relationship between most of them with grouping of specific taxonomic units was observed.