Open AccessRapid identification of S accharomyces eubayanus and its hybrids
Author(s) -
Pengelly Reuben J.,
Wheals Alan E.
Publication year - 2013
Publication title -
fems yeast research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.991
H-Index - 92
eISSN - 1567-1364
pISSN - 1567-1356
DOI - 10.1111/1567-1364.12018
Subject(s) - biology , saccharomyces , hybrid , primer (cosmetics) , genetics , phylogenetic tree , saccharomyces cerevisiae , gene , botany , chemistry , organic chemistry
The S accharomyces species S accharomyces eubayanus was recently discovered in P atagonia. Genomic, genetic and phylogenetic data all suggest that it is one of the two parents of the hybrid yeast S . pastorianus ( S . cerevisiae being the other). S accharomyces eubayanus genomes can also be found in strains of the hybrid species S . bayanus . Here, we describe a novel pair of PCR primers targeted against the S . eubayanus FSY1 gene that will specifically detect S . eubayanus and hybrids containing this allele of the gene. The primer pair can be used to identify the species using a rapid, inexpensive colony PCR method suitable for a preliminary identification of wild isolates.