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Manual determination of plant phenotypic properties such as plant architecture, growth, and health is very time consuming and sometimes destructive. Automatic image analysis has become a popular approach. This research aims to identify the position (and number) of leaves from a temporal sequence of high-quality indoor images consisting of multiple views, focussing in particular of images of maize. The procedure used a segmentation on the images, using the convex hull to pick the best view at each time step, followed by a skeletonization of the corresponding image. To remove skeleton spurs, a discrete skeleton evolution pruning process was applied. Pre-existing statistics regarding maize development was incorporated to help differentiate between true leaves and false leaves. Furthermore, for each time step, leaves were matched to those of the previous and next three days using the graph-theoretic Hungarian algorithm. This matching algorithm can be used to both remove false positives, and also to predict true leaves, even if they were completely occluded from the image itself. The algorithm was evaluated using an open dataset consisting of 13 maize plants across 27 days from two different views. The total number of true leaves from the dataset was 1843, and our proposed techniques detects a total of 1690 leaves including 1674 true leaves, and only 16 false leaves, giving a recall of 90.8%, and a precision of 99.0%.

A Novel Technique Combining Image Processing, Plant Development Properties, and the Hungarian Algorithm, to Improve Leaf Detection in Maize