Open AccessCrystallization and preliminary crystallographic analysis of the chimeric protein LKB1–14‐3‐3ζ
Author(s) -
Ding Sheng,
Zhou Ruiqing,
Zhu Yaqin
Publication year - 2013
Publication title -
acta crystallographica section f
Language(s) - English
Resource type - Journals
ISSN - 1744-3091
DOI - 10.1107/s1744309113029230
Subject(s) - chimera (genetics) , crystallization , crystallography , fusion protein , protein crystallization , chemistry , biophysics , biology , biochemistry , recombinant dna , gene , organic chemistry
LKB1, a tumour suppressor, plays key roles in cell polarity, cell growth and energy metabolism. 14‐3‐3 proteins bind to LKB1 and suppress its functions. A chimera containing 14‐3‐3ζ and its binding region in LKB1 was constructed and the chimeric protein LKB1–14‐4‐3ζ was purified and crystallized. The crystal of LKB1–14‐4‐3ζ diffracted to 2.9 Å resolution and belonged to space group R 32, with unit‐cell parameters a = b = 130.262, c = 264.960 Å. Structure determination and refinement are in progress.