Open AccessCrystallization and preliminary X‐ray analysis of human leukocyte cell‐derived chemotaxin 2 (LECT2)
Author(s) -
Zheng Hai,
Miyakawa Takuya,
Sawano Yoriko,
Yamagoe Satoshi,
Tanokura Masaru
Publication year - 2013
Publication title -
acta crystallographica section f
Language(s) - English
Resource type - Journals
ISSN - 1744-3091
DOI - 10.1107/s1744309113003758
Subject(s) - crystallization , crystallography , peg ratio , solvent , chemistry , crystal (programming language) , ammonium sulfate , materials science , biochemistry , chromatography , organic chemistry , finance , computer science , economics , programming language
Human leukocyte cell‐derived chemotaxin 2 (LECT2) is a chemotactic factor for neutrophils that plays multifunctional roles in liver regeneration, regulation of neuritic development and proliferation of chondrocytes and osteoblasts. In addition, the C‐terminal region of LECT2 belongs to the zinc metalloendopeptidase M23 (PF01551) family. Purified LECT2 was crystallized using the sitting‐drop vapour‐diffusion method at 293 K. Crystals of selenomethionine‐substituted LECT2 that diffracted X‐rays to 1.94 Å resolution were obtained using a reservoir solution consisting of 0.2 M ammonium sulfate, 0.1 M HEPES pH 7.5, 25%( w / v ) PEG 8000. The crystal belonged to space group P 2 1 2 1 2 1 , with unit‐cell parameters a = 59.4, b = 63.5, c = 64.0 Å. The calculated Matthews coefficient ( V M = 2.10 Å 3 Da −1 , solvent content 40%) indicates that the crystal consists of two molecules per asymmetric unit.