Open AccessExpression, purification, crystallization and preliminary X‐ray crystallographic analysis of Zucchini from Drosophila melanogaster
Author(s) -
Fukuhara Satoshi,
Nishimasu Hiroshi,
Bonnefond Luc,
Matsumoto Naoki,
Ishitani Ryuichiro,
Nureki Osamu
Publication year - 2012
Publication title -
acta crystallographica section f
Language(s) - English
Resource type - Journals
ISSN - 1744-3091
DOI - 10.1107/s1744309112038936
Subject(s) - piwi interacting rna , transposable element , biogenesis , drosophila melanogaster , biology , melanogaster , escherichia coli , heterochromatin , genetics , microbiology and biotechnology , genome , gene , chromosome
PIWI‐interacting RNAs (piRNAs) bind PIWI proteins and silence transposons to maintain the genomic integrity of germ cells. Zucchini (Zuc), a phospholipase D superfamily member, is conserved among animals and is implicated in piRNA biogenesis. However, the underlying mechanism by which Zuc participates in piRNA biogenesis remains elusive. Drosophila melanogaster Zuc (DmZuc) was expressed in Escherichia coli , purified and crystallized. X‐ray diffraction data were collected to 1.75 Å resolution. The crystal belonged to space group P 2 1 , with unit‐cell parameters a = 55.0, b = 71.2, c = 56.3 Å, β = 107.9°.