Open AccessCrystallization and preliminary X‐ray diffraction analysis of rat autotaxin
Author(s) -
Day Jacqueline E.,
Hall Troii,
Pegg Lyle E.,
Benson Timothy E.,
Hausmann Jens,
Kamtekar Satwik
Publication year - 2010
Publication title -
acta crystallographica section f
Language(s) - English
Resource type - Journals
ISSN - 1744-3091
DOI - 10.1107/s1744309110030228
Subject(s) - crystallization , autotaxin , x ray , x ray crystallography , materials science , diffraction , crystallography , chemistry , optics , physics , biochemistry , organic chemistry , lysophosphatidic acid , receptor
Rat autotaxin has been cloned, expressed, purified to homogeneity and crystallized via hanging‐drop vapour diffusion using PEG 3350 as precipitant and ammonium iodide and sodium thiocyanate as salts. The crystals diffracted to a maximum resolution of 2.05 Å and belonged to space group P 1, with unit‐cell parameters a = 53.8, b = 63.3, c = 70.5 Å, α = 98.8, β = 106.2, γ = 99.8°. Preliminary X‐ray diffraction analysis indicated the presence of one molecule per asymmetric unit, with a solvent content of 47%.