Open AccessCrystallization and preliminary X‐ray analysis of the tumour necrosis factor α–tumour necrosis factor receptor type 2 complex
Author(s) -
Mukai Yohei,
Nakamura Teruya,
Yoshioka Yasuo,
Tsunoda Shinichi,
Kamada Haruhiko,
Nakagawa Shinsaku,
Yamagata Yuriko,
Tsutsumi Yasuo
Publication year - 2009
Publication title -
acta crystallographica section f
Language(s) - English
Resource type - Journals
ISSN - 1744-3091
DOI - 10.1107/s1744309109004461
Subject(s) - necrosis , crystallization , tumor necrosis factor alpha , materials science , cancer research , pathology , chemistry , medicine , organic chemistry
Tumour necrosis factor receptor type 2 (TNFR2, TNFRSF1B) is an essential receptor for various host‐defence functions of tumour necrosis factor α (TNF). As part of studies to determine the structure of TNFR2, the formation, crystallization and preliminary X‐ray diffraction analysis of the TNF–TNFR2 complex are described. The TNF–TNFR2 complex, which comprises one TNF trimer and three TNFR2 monomers, was confirmed and purified by size‐exclusion chromatography. Crystals of the TNF–TNFR2 complex were obtained using polyethylene glycol 3350 as a precipitant. The crystal belonged to space group P 2 1 2 1 2 1 , with unit‐cell parameters a = 74.5, b = 117.4, c = 246.8 Å. Assuming the presence of two TNF–TNFR2 complexes in the asymmetric unit, the Matthews coefficient V M was 2.49 Å 3 Da −1 and the solvent content of the crystal was 50.7%. The crystal diffracted to 2.95 Å resolution.