Open AccessCrystallization and preliminary X‐ray analysis of the NKX2.5 homeodomain in complex with DNA
Author(s) -
Genis Caroli,
Scone Peyton,
Kasahara Hideko,
Nam HyunJoo
Publication year - 2008
Publication title -
acta crystallographica section f
Language(s) - English
Resource type - Journals
ISSN - 1744-3091
DOI - 10.1107/s1744309108033447
Subject(s) - crystallization , homeobox , x ray , dna , crystallography , materials science , chemistry , genetics , biology , physics , gene , optics , transcription factor , organic chemistry
As part of an effort to elucidate the molecular basis for the pathogenesis of NKX2.5 mutations in congenital heart disease using X‐ray crystallography, the NKX2.5 homeodomain has been crystallized in complex with a specific DNA element, the −242 promoter region of atrial natriuretic factor. Crystals of the homeodomain–DNA complex diffracted X‐rays to 1.7 Å resolution and belonged to space group P 6 5 , with unit‐cell parameters a = b = 71.5, c = 94.3 Å. The asymmetric unit contained two molecules of the NKX2.5 homeodomain and one double‐stranded oligonucleotide.