Open AccessCloning, expression, purification, crystallization and preliminary X‐ray diffraction analysis of 2‐keto‐3‐deoxy‐6‐phosphogluconate (KDPG) aldolase from Streptococcus suis serotype 2
Author(s) -
Zhang Qiangmin,
Gao Feng,
Qi Jianxun,
Cheng Hao,
Liu Yiwei,
Gao George F.
Publication year - 2008
Publication title -
acta crystallographica section f
Language(s) - English
Resource type - Journals
ISSN - 1744-3091
DOI - 10.1107/s1744309108028340
Subject(s) - crystallization , resolution (logic) , serotype , cloning (programming) , crystallography , aldolase a , chemistry , x ray crystallography , microbiology and biotechnology , diffraction , biology , biochemistry , enzyme , physics , optics , organic chemistry , artificial intelligence , computer science , programming language
2‐Keto‐3‐deoxy‐6‐phosphogluconate (KDPG) adolase from pathogenic Streptococcus suis serotype 2 was crystallized using the hanging‐drop vapour‐diffusion method at 291 K. X‐ray diffraction data were collected to 2.8 Å resolution. The crystal belonged to space group R 32, with unit‐cell parameters a = b = 126.4, c = 415.9 Å, α = β = 90, γ = 120°. Assuming the presence of six molecules in the asymmetric unit gave a V M value of 2.32 Å 3 Da −1 and a solvent content of 47.12%.