Open AccessCrystallization and X‐ray analysis of the Schistosoma mansoni guanidino kinase
Author(s) -
Awama Ayman M.,
Paracuellos Patricia,
Laurent Sabine,
Dissous Colette,
Marcillat Olivier,
Gouet Patrice
Publication year - 2008
Publication title -
acta crystallographica section f
Language(s) - English
Resource type - Journals
ISSN - 1744-3091
DOI - 10.1107/s1744309108025979
Subject(s) - schistosoma mansoni , arginine kinase , monoclinic crystal system , crystallography , kinase , crystallization , chemistry , amino acid , microbiology and biotechnology , biology , biochemistry , arginine , crystal structure , schistosomiasis , immunology , helminths , organic chemistry
The 716‐amino‐acid guanidino kinase from the parasitic flatworm Schistosoma mansoni results from the fusion of two guanidino kinase subunits. Crystals of this 80 kDa protein have been obtained in the monoclinic space group P 2 1 , with unit‐cell parameters a = 52.7, b = 122.1, c = 63.2 Å, β = 108.5°. Synchrotron data were collected to 2.8 Å resolution on ESRF beamline ID29. The structure was solved by the molecular‐replacement method, using the 357‐amino‐acid structure of the arginine kinase from Trypanosoma cruzi as the search model.