Open AccessCrystallization and X‐ray diffraction analysis of a novel immune‐type receptor from Ictalurus punctatus and phasing by selenium anomalous dispersion methods
Author(s) -
Ostrov David A.,
Hernández Prada José A.,
Haire Robert N.,
Can John P.,
Magis Andrew T.,
Bailey Kate,
Litman Gary W.
Publication year - 2007
Publication title -
acta crystallographica section f
Language(s) - English
Resource type - Journals
ISSN - 1744-3091
DOI - 10.1107/s1744309107054231
Subject(s) - ictalurus , crystallization , diffraction , crystallography , selenium , chemistry , x ray crystallography , materials science , analytical chemistry (journal) , catfish , chromatography , biology , physics , optics , organic chemistry , fishery , fish <actinopterygii>
X‐ray diffraction data from crystals of a novel immune‐type receptor (NITR10 from the catfish Ictalurus punctatus ) were collected to 1.65 Å resolution and reduced to the primitive hexagonal lattice. Native and selenomethionine derivatives of NITR10 crystallized under different conditions yielded P 3 1 21 crystals. SeMet NITR10 was phased to a correlation coefficient of 0.77 by SAD methods and experimental electron‐density maps were calculated to 1.65 Å. Five NITR10 molecules are predicted to be present in the asymmetric unit based on the Matthews coefficient.