Crystallization and preliminary crystallographic analysis of an octaketide‐producing plant type III polyketide synthase | Zendy

Morita Hiroyuki | Zendy; Kondo Shin | Zendy; Kato Ryohei | Zendy; Wanibuchi Kiyofumi | Zendy; Noguchi Hiroshi | Zendy; Sugio Shigetoshi | Zendy; Abe Ikuro | Zendy; Kohno Toshiyuki | Zendy

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Crystallization and preliminary crystallographic analysis of an octaketide‐producing plant type III polyketide synthase

Author(s) -

Morita Hiroyuki,

Kondo Shin,

Kato Ryohei,

Wanibuchi Kiyofumi,

Noguchi Hiroshi,

Sugio Shigetoshi,

Abe Ikuro,

Kohno Toshiyuki

Publication year - 2007

Publication title -

acta crystallographica section f

Language(s) - English

Resource type - Journals

ISSN - 1744-3091

DOI - 10.1107/s1744309107047069

Subject(s) - atp synthase , crystallography , molecule , chemistry , polyketide synthase , crystallization , stereochemistry , polyketide , enzyme , biochemistry , biosynthesis , organic chemistry

Octaketide synthase (OKS) from Aloe arborescens is a plant‐specific type III polyketide synthase that produces SEK4 and SEK4b from eight molecules of malonyl‐CoA. Recombinant OKS expressed in Escherichia coli was crystallized by the hanging‐drop vapour‐diffusion method. The crystals belonged to space group I 422, with unit‐cell parameters a = b = 110.2, c = 281.4 Å, α = β = γ = 90.0°. Diffraction data were collected to 2.6 Å resolution using synchrotron radiation at BL24XU of SPring‐8.

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