Open AccessCrystallization and preliminary X‐ray analysis of the C‐terminal RNase III domain of human Dicer
Author(s) -
Takeshita Daijiro,
Zenno Shuhei,
Lee Woo Cheol,
Nagata Koji,
Saigo Kaoru,
Tanokura Masaru
Publication year - 2006
Publication title -
acta crystallographica section f
Language(s) - English
Resource type - Journals
ISSN - 1744-3091
DOI - 10.1107/s174430910600889x
Subject(s) - dicer , crystallization , terminal (telecommunication) , domain (mathematical analysis) , crystallography , rnase p , materials science , chemistry , rna , biochemistry , computer science , mathematics , gene , small interfering rna , organic chemistry , telecommunications , mathematical analysis
Human Dicer protein contains two RNase III domains (RNase IIIa and RNase IIIb) which are involved in the production of short interfering RNAs (siRNAs). The C‐terminal RNase III domain (RNase IIIb) of human Dicer was expressed, purified and crystallized by the sitting‐drop vapour‐diffusion method. The crystals belonged to space group C 222 1 , with unit‐cell parameters a = 88.6, b = 199.7, c = 119.6 Å, and diffracted X‐rays to 2.0 Å resolution. The asymmetric unit contained three molecules of the RNase IIIb and the solvent content was 67%.