Open AccessCrystallization and preliminary crystallographic analysis of the site‐specific DNA nickase Nb.BspD6I
Author(s) -
Kachalova Galina S.,
Rogulin Eugeny A.,
Artyukh Rimma I.,
Perevyazova Tatyana A.,
Zheleznaya Ludmila A.,
Matvienko Nickolay I.,
Bartunik Hans D.
Publication year - 2005
Publication title -
acta crystallographica section f
Language(s) - English
Resource type - Journals
ISSN - 1744-3091
DOI - 10.1107/s174430910500309x
Subject(s) - crystallization , crystallography , crystal (programming language) , resolution (logic) , molecule , dna , solvent , materials science , peg ratio , x ray crystallography , crystal structure , diffraction , chemistry , biochemistry , organic chemistry , physics , computer science , artificial intelligence , economics , finance , optics , programming language
Crystals of site‐specific DNA nickase Nb.BspD6I (of molecular weight 70.8 kDa) have been grown at 291 K using PEG 8000 as precipitant. The diffraction pattern of the crystal extends to 3.3 Å resolution at 100 K. The crystal belongs to space group P 2 1 , with unit‐cell parameters a = 57.76, b = 90.67, c = 71.71, β = 110.1°. There is one molecule in the asymmetric unit and the solvent content is estimated to be 53% by volume.
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