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The title compound, digoxigenone, C 23 H 30 O 5 ·H 2 O, was biotransformed from digoxigenin. In the crystal, inter­molecular O—H⋯O hydrogen bonds contribute to the formation of a three-dimensional supra­molecular structure. The title compound has three fused six-membered rings ( A , B , C ) and two non-fused five-membered rings ( D , E ). As in other structures, compound nucleus has a  cis - trans - cis  conformation for the  A - B , B - C , C - D  ring junctions with  rings  A ,  B  and  C  exhibiting chair conformations.

12β,14-Dihydroxy-3-oxo-5β,20(22)-cardenolide monohydrate