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In the title anhydro­scymnol tetra­acetate, C 35 H 54 O 9 , the fused chair conformation of the cyclo­hexane  A / B  ring junction is  cis  with a 5β- H  configuration. The compound has a trimethyl­ene oxide ring at position 24,26 and four acetate groups at the 3α,7α,12α,27 positions.

(20R,24R,25S)-3α,7α,12α,27-Tetraacetoxy-24,26-epoxy-5β-cholestane