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25th European Crystallographic Meeting, ECM 25, İstanbul, 2009 Acta Cryst. (2009). A65, s 32 Page s 32 crystal-like objects are biomolecular and identification of crystals in crystallisation set ups. The outcome is an advanced instrumentation, which combines a imaging and dynamic light scattering (DLS) system for routine measurements in drops in standard multi-well plates used in protein crystallization as well as geltube crystallisation experiments and crystallization experiments kept under oil, allowing a systematic optimization of the crystallization process. Furthermore the system is most suitable for upscaling crystallization conditions obtained in nanodrops prepared by crystallization robots towards higher volumes yielding more X-ray suitable crystals. The system was tested with several standard proteins and found to be of high value for rapid identification of good crystallization conditions. A relationship between the rate of protein-aggregate-size increase and the probability of crystal formation was observed and results will be presented accordingly. All together the newly designed hardware has several advantages: no additional pipetting is necessary to perform measurements; the crystallisations process can be monitored online in situ, without interruption; measurements can be taken from even small volumes. This new DLS technique has been adapted to an automated CCD-camera-based plate-screening system (Spectro-Imager 501) allowing monitoring and evaluation of the entire process of crystallisation in an automated way. The data obtained provide information to understand in detail the process of crystal growth. Finally we will also describe in detail a method to support the identification of protein crystals, exploiting the fact that most proteins and other biomolecules fluoresce when illuminated with UV light. The Spectro-Imager 501® is a completely new instrumental design incorporating all of the techniques described above in one device. Images taken from various droplets/set ups will be presented along with corresponding DLS measurements

A crystallization platform enabling automatedin situdiffraction screening