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Plant chloroplast genes encode rRNAs, tRNAs, and proteins for their own transcription and translation systems. Plant chloroplast ribosomes, which are a part of the translational apparatus, are prokaryotic like, 70s in size, and comprise 16S, 23S, 5S, and 4.55 rRNAs and about 58 to 62 different ribosomal protein species (Subramanian, 1985). Nineteen to 20 different ribosomal protein genes that code 11 or 12 proteins of the small ribosomal subunit and 8 proteins of the large subunit are located in the chloroplast genome (Shinozaki et al., 1986b). Here we report the full-length sequence of a cDNA encoding the potato (Solanum tuberosum L.) chloroplast ribosoma1 protein S16 (cp rpsZ6) (Table I). The deduced amino acid sequence of cp rpsl6 gene is over 80% conserved among higher plants, but is less than 30% conserved between plants and Escherichia coli (Kanakari et al., 1992). Stern et al. (1988) reported that E. coli ribosomal protein S16 was associated with other ribosomal proteins (S4, S8, and S20) to facilitate the binding and assembly of the 16s rRNA into the 30s subunit. Using conventional differential screening techniques, we obtained several early tuber cDNA clones from a 4-d axillary bud-tuber AZAP library. One of these clones, designated p24-2, contained an approximately 1.6-kb insert and was sequenced in its entirety. The 1561-bp sequence showed high homology with known chloroplast rps l6 genes, which code for chloroplast ribosomal protein S16 of tobacco, rice, mustard, and maize (Shinozaki et al., 1986a, 1986b; Hiratsuka et al., 1989; Neuhaus et al., 1989; Kanakari et al., 1992, respectively). Based on the DNA sequence similarity and its amino acid sequence analysis, we concluded that the cDNA p24-2 was the potato chloroplast rps16 gene. This potato cp rpsl6 gene is located between the trnK gene for tRNALyS (UUU) and the trnQ gene for tRNAG'" (UUG) on the same strand in the chloroplast genome. The 5' untranslated sequence contains an AT-rich region of 239 nucleotides and a putative ribosome-binding sequence element 5'-AGGA-3' that is located five nucleotides upstream from the initiation codon, ATG. Two reading

Nucleotide Sequence of a cDNA for the Potato (Solanum tuberosum L.) Chloroplast Ribosomal Protein S16