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Using a data sample corresponding to an integrated luminosity of $2.93\,\rmfb^{-1}$ taken at a center-of-mass energy of 3.773\,GeV with the BESIIIdetector operated at the BEPCII collider, we perform an analysis of thesemi-leptonic decays $D^{0(+)}\to \pi^{-(0)}\mu^+\nu_\mu$. The branchingfractions of $D^0\to \pi^-\mu^+\nu_\mu$ and $D^+\to \pi^0\mu^+\nu_\mu$ aremeasured to be $(0.272 \pm 0.008_{\rm stat.} \pm 0.006_{\rm syst.})\%$ and$(0.350 \pm 0.011_{\rm stat.} \pm 0.010_{\rm syst.})\%$, respectively, wherethe former is of much improved precision compared to previous results and thelatter is determined for the first time. Using these results along withprevious BESIII measurements of $D^{0(+)}\to \pi^{-(0)}e^+\nu_e$, we calculatethe branching fraction ratios to be ${\mathcal R}^0\equiv {\mathcalB}_{D^{0}\to \pi^{-}\mu^+\nu_\mu}/{\mathcal B}_{D^{0}\to\pi^{-}e^+\nu_e}=0.922\pm 0.030_{\rm stat.}\pm0.022_{\rm syst.}$ and ${\mathcalR}^+\equiv {\mathcal B}_{D^{+}\to \pi^{0}\mu^+\nu_\mu}/{\mathcal B}_{D^{+}\to\pi^{0}e^+\nu_e}=0.964\pm 0.037_{\rm stat.}\pm0.026_{\rm syst.}$, which arecompatible with the theoretical expectation of lepton flavor universalitywithin $1.7\sigma$ and $0.5\sigma$, respectively. We also examine the branchingfraction ratios in different four-momentum transfer square regions, and find nosignificant deviations from the standard model predictions.

Measurement of the Branching Fraction For the Semi-Leptonic Decays $D^{0(+)}\to \pi^{-(0)}\mu^+\nu_\mu$ and Test of Lepton Flavor Universality