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We demonstrate thermally limited force spectroscopy using a probe formed by adielectric microsphere optically trapped in water near a dielectric surface. Weachieve force resolution below 1 fN in 100 s, corresponding to a 2 {\AA} rmsdisplacement of the probe. Our measurement combines a calibrated evanescentwave particle tracking technique and a lock-in detection method. We demonstratethe accuracy of our method by measurement of the height-dependent force exertedon the probe by an evanescent wave, the results of which are in agreement withMie theory calculations.

Subfemtonewton Force Spectroscopy at the Thermal Limit in Liquids