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We numerically study the dynamics of run-and-tumble particles confined in twochambers connected by thin channels. Two dominant dynamical behaviors emerge:(i) an oscillatory pumping state, in which particles periodically fill the twovessels and (ii) a circulating flow state, dynamically maintaining a nearconstant population level in the containers when connected by two channels. Wedemonstrate that the oscillatory behaviour arises from the combination of anarrow channel, preventing bacteria reorientation, and a density dependentmotility inside the chambers.

Self-Sustained Density Oscillations of Swimming Bacteria Confined in Microchambers