Open AccessConformation and Dynamics of DNA Confined in Slitlike Nanofluidic Channels
Author(s) -
Douwe Jan Bonthuis,
Christine Meyer,
Derek Stein,
Cees Dekker
Publication year - 2008
Publication title -
physical review letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.688
H-Index - 673
eISSN - 1079-7114
pISSN - 0031-9007
DOI - 10.1103/physrevlett.101.108303
Subject(s) - radius of gyration , radius , gyration , relaxation (psychology) , scaling , anisotropy , materials science , condensed matter physics , hydrodynamic radius , molecular dynamics , physics , molecular physics , polymer , nanotechnology , optics , nanoparticle , quantum mechanics , geometry , psychology , social psychology , computer security , mathematics , computer science , dynamic light scattering , composite material
Using laser fluorescence microscopy, we study the shape and dynamics of individual DNA molecules in slitlike nanochannels confined to a fraction of their bulk radius of gyration. With a confinement size spanning 2 orders of magnitude, we observe a transition from the de Gennes regime to the Odijk regime in the scaling of both the radius of gyration and the relaxation time. The radius of gyration and the relaxation time follow the predicted scaling in the de Gennes regime, while, unexpectedly, the relaxation time shows a sharp decrease in the Odijk regime. The radius of gyration remains constant in the Odijk regime. Additionally, we report the first measurements of the effect of confinement on the shape anisotropy
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