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We propose and demonstrate the sequential initialization, optical control,and read-out of a single spin trapped in a semiconductor quantum dot. Hole spinpreparation is achieved through ionization of a resonantly excitedelectron-hole pair. Optical control is observed as a coherent Rabi rotationbetween the hole and charged exciton states, which is conditional on theinitial hole spin state. The spin-selective creation of the charged excitonprovides a photocurrent read-out of the hole spin state.

Fast optical preparation, control and read-out of single quantum dot spin